What does it mean for a peptide to be lyophilized?
Lyophilization is a low-temperature dehydration process that converts a liquid compound into a dry powder. It extends the compound's shelf life up to 2 years, maintains chemical stability, facilitates transport, and reduces degradation from moisture or heat.
What is bacteriostatic water and why use it?
Bacteriostatic water contains 0.9% benzyl alcohol. It allows multi-dose use, maintains sterility for weeks, is compatible with most peptides, and has a neutral pH.
Step-by-step guide: how to reconstitute a lyophilized peptide
- Prepare your work area with 70% alcohol.
- Calculate the bacteriostatic water volume using the calculator.
- Disinfect the septum with an alcohol swab.
- Draw the bacteriostatic water with the syringe.
- Inject slowly along the vial wall — never directly onto the powder.
- Do not shake — gently swirl until dissolved.
- The solution should be clear or slightly opalescent.
- Refrigerate at 2-8°C immediately afterward.
Common mistakes
- Shaking vigorously → denaturation.
- Using distilled water → bacterial contamination.
- Injecting directly onto the powder → degradation.
- Storing reconstituted peptide at room temperature → accelerated degradation.
Post-reconstitution storage
- Refrigerated 2-8°C: 4-6 weeks.
- Frozen -20°C: 3-6 months.
- Room temperature: not recommended.
Conclusion
Proper reconstitution ensures the peptide maintains its chemical integrity. BioPeptideX offers bacteriostatic water available in the catalog.